The Oldham Athletic youth team, sponsored by the Safeguard Group, were beaten 4-1 on Saturday as Preston North End ended their ten game winning streak.
Devante Jacobs' penalty had levelled the game just before the break after Preston took an early lead but three second half goals eventually saw them run out comfortable winners, with Latics finishing the game with nine men.
After manager Tony Philliskirk made all three of his substitutes, midfielder Alex Reid and defender Callum Etches both picked up injuries and were forced to leave the field.
The visitors made their extra men advantage count and came away with the victory.
Speaking about the game, Philliskirk admitted: "Sometimes you have to hold your hands up and say that Preston were better than us on the day.
"I'm not going to criticise my players because a lot happened in the second half. In hindsight, I should not have made the substitutions so early on but I thought I would try something and it backfired.
"I said to the players at the end to rip the game up and throw it in the bin. We will learn from it and make it better next weekend.
"Preston came and made it tough for us and everything that could have gone wrong in the second half did go wrong. Preston are a strong and physical team though. They stopped us playing our game but fair play to them for that."
The result ended Latics' unbelievable winning streak but the gaffer is now looking for a reaction from his players.
"The players had won ten games in a row up until that point but I'm looking forward to how they react now as we face Port Vale next week who again are a strong physical side," said Philliskirk.
The Oldham Athletic academy are sponsored by the Safeguard Group and you can access their website by clicking here