A frantic second-half saw goals from Christopher Missilou and Johan Branger-Engone cancelled out by Matthew Lund and Kevin van Veen to see the third consecutive 2-2 draw in the league.
Laurent Banide made one change to the starting eleven from the weekend with Chris Eagles making a return for Mohamad Sylla.
And it was Oldham who started the brighter under the night sky in an uneventful opening 45-minutes.
A succession of corners for Latics in the opening 10 minutes were all cleared at the first attempt by the hosts.
The Iron’s first attempt came five minutes later when David Wheater lost possession. As Abo Eisa lined up a shot, the Oldham Captain make amends quickly by tackling cleanly in the box to stop the shot.
The Dutchman for Scunthorpe, Kevin van Veen then got in on the act, with a curling effort going over the crossbar on the 20-minute mark.
Both sides continued to create half chances with it ending goalless at the interval.
As the second period begun it was Oldham Athletic who stunned Glanford Park with two quick goals after van Veen hit the upright from a dead ball on the hour mark.
The first Latics goal came courtesy of Missilou. Breaking free on the left, it was the midfielder who calmly slotted home across goal to put the tangerine team in front.
It was only just over 60 seconds later before Banide’s team doubled their lead. On his 50th appearance for the club, Branger received the ball in space on the edge of the box in the centre, unleashing a fast shot that squirmed under Rory Watson in the home goal.
Unfortunately, Latics would squander a two-goal lead again, conceding the first less than five minutes from taking a commanding position.
The defender Lund found the ball in his lap at the far post and from close range guided it past Woods to change the complexion of the game.
With Paul Hurst’s side searching for that all-important equaliser, it was the substitute who nearly made an instant impact.
Ryan Colclough who would become a threat for the home side, headed over from close range.
However, Scunthorpe would find their second on the 76th minute. With van Veen receiving the ball on the left, he skipped past Jonny Smith before firing across goal.
As the game came to a close, both teams had chances to find the winner with a flurry of yellow cards also following.
The most notable for Latics came when Azankpo’s back post header was cleared off the line acrobatically with ten minutes remaining.
Five minutes after and Colclough beat two player before finding his shot deflected at close range, the ball spinning away and brushing the far post.
But both teams would have to settle with a point, with Latics obviously feeling deflated again after doing so well to take the lead.
Scunthorpe United: Watson, Lund, Songo’o, van Veen, Perch, Gillead, McArdle (Capt), McGahey, Brown, Eisa (72' Colclough), Proctor.
Oldham Athletic: Woods, Iacovitti, Branger-Engone (75' Smith), Morais, Maouche (90' Mills), Missilou, Fage, Azankpo, Wheater (Capt), Eagles (65' Sylla), Hamer.
Attendance: 3,020
Referee: Darren Drysdale